The calcium, essential for the human body, is kept at levels from 8.5 to 10.5 mg / dl. Higher levels (hypercalcemia) are detected in the menopause, when the rate of reabsorption increases regarding the reduction of ovarian estrogen production and, consequently, more calcium is needed to manage the reduction in the efficiency of the use of mineral obtained from food. It was made an observational and descriptive study, which purpose was to determine the serum calcium using photocolorimetry in 45-55 years old women. According to the aim, 201 blood samples from patients belonging to urban area parishes of the city of Cuenca were analyzed, in order to identify the relationship between hypercalcemia and hormonal alteration these women. The processing of the samples was performed at the Laboratory of the” Vicente Corral Moscoso” Hospital, following biosafety standards and quality control. Next, the data was entered to SPSS and Excel software, getting these results: The 50.2% of women surveyed are over 50 years old. 56.7% of these women have a rate of calcium between 9 and 10.9 mg / dl, while 33.3% have values higher than 11 mg / dl relating it to a study carried out in Costa Rica with outpatient population in 45 healthy adult women randomly selected, without apparent disorders of calcium metabolism, in which was obtained serum calcium between 8.6 - 10.3 mg / dl.