The purpose of this study was to examine the knowledge and attitudes towards infant feeding practices amongst HIV-infected women in Southern Botswana. A questionnaire-based survey was undertaken at Baylor Family Model Clinic in Gaborone. Subjects included postnatal women with HIV infection (Group 1, n=120) and antenatal women with HIV infection (Group 2, n=80). Advantages of breastfeeding and formula feeding according to several characteristics (convenience, cleanliness, cheapness and safety) were rated using a four-point (0-3) scale. Overall, breastfeeding was rated much higher (11.4/12) than Formula feeding (6.3/12) (p<0, 0005). Formula feeding rating was highest among postnatal women with HIV infection (6.8/12); however, it was lower than the rating for breastfeeding (11.3/12). The majority of women with HIV infection were either Formula feeding (Group 1, 92%) or intended to formula feed (Group 2, 78%) their infants for the first 6 months. All the women, despite their known HIV seropositive status still considered breastfeeding to be more advantageous than formula feeding. However, once women with HIV infection were informed of the reduced risk of HIV transmission through breastfeeding while on anti-retroviral therapy, they should be better equipped to make their own decisions to follow the Botswana Ministry of Health’s 2011 PMTCT recommendations.