This study, first of its kind in Princess Marina Hospital and conducted from 12th to 19th of January 2012, was aimed at determining the knowledge, attitudes and perception of routine HIV testing by the industrial class workers of Princess Marina Hospital. This study has shown that awareness of HIV and AIDS and HIV testing is universal in the study population. It has alsoshown that routine HIV testing is not practiced as it should be at the staff clinic of Princess Marina Hospital. It has revealed that a significant number of respondents have knowledge gaps with regards to the transmission of HIV and this could lead to risky behaviors and the resulting spread of HIV. Voluntary Counseling and Testing Centres seem to be the preferred HIV testing places. The study has also determined that HIV test results taking long, lack of confidentiality, fear of stigma and discrimination were the most important reasons for not testing at Princess Marina Hospital. Appropriate measures need to be taken to correct identified gaps and ensure a successful implementation of routine HIV testing in order to strengthen the fight against HIV/AIDS at the workplace.