Improper antibiotic usage in terms of overuse and misuse is considered the most important factor
promoting the emergence, selection and dissemination of antibiotic resistant microorganisms in veterinary and
human medicine. Enteric bacterial isolates from marketed fish in fifteen locations in Ibadan metropolis were
randomly sampled to examine for their antibiotic resistance to some commonly used antibiotics. Bacteriological
examination of collected fish intestine and gills was done using standard methods, followed by in-vitro antibiotic
sensitivity test by agar gel diffusion technique. Three enteric bacteria; Escherichia coli, Staphylococcus aureus and
Klebsiella pneumoniae that were multi-drug resistant to different combinations of aminoglycosides, cephalosporins
and fluoroquinolones group of drugs were isolated. The occurrence of multiple antibiotic resistances among the
enteric bacterial isolates from sampled marketed fish in the study area indicate a potential public health risk as it has
been shown that animals including fish can act as vectors of bacteria. It is suggested that the prophylactic use of
antibiotics may be a predisposing factor in the occurrence of antibiotic resistance among enteric bacteria in fish.
Therefore, it is proposed that the application of antimicrobial drugs should be strictly controlled in tropical
aquaculture to prevent the dissemination of antibiotic-resistant bacteria.