Knowledge and attitudes of children towards wildlife and the environment were assessed through questionnaires given to 366 children in 9 schools in southwest Guyana. Children’s responses revealed that they had a general knowledge of wildlife but knew few details about specific species. Respondents thought that wildlife was important but were nonetheless tolerant of several forms of environmental exploitation. Visits by conservation organizations, Conservation International and Foster Parrots, were shown to increase appreciation of wildlife and the need to set up more protected areas, but unless programmes were concentrated and sustained they had little effect on attitudes towards environmental utilization and exploitation. Experience of the natural world in terms of owning a domestic animal or pet, having visited a zoo, or being a member of a wildlife club had little impact on children’s knowledge of wildlife and did not change attitudes to utilization and exploitation. Surprisingly, Guyanese children did not have particularly positive views about classic flagship species, such as jaguars, giant anteaters and tapirs, when compared to other species. Our findings suggest that zoos, wildlife clubs and conservation organizations could enhance the dissemination of their message through making more frequent and sustained visits, imparting more detailed knowledge, and exposing children to the potential dangers of utilization and exploitation; also that conservation organizations should reassess their use of standard flagship species in South America.