Toxoplasma gondii is obligate coccidian zoonotic parasite. Felidae family is definitive and wide ranges of warm-blooded vertebrates
are intermediate hosts for the parasite. Rodents are measured as an important source of T. gondii infection for the definitive host.
Thus, this study aimed to investigate Toxoplasm infection in rodents of Ahvaz district, southwest of Iran. A total of 100 rodents (73
Rattus norvegicus, 21 Rattus rattus, and 6 Mus musculus) were collected and studied by GRA6PCR and mouse bioassay. The finding
indicated that 6 out of 100 (6%) and 2 out of 100 (2%) samples were positive by PCR and mouse bioassay, respectively. The results
show notable chronic infection in the rodent and potential transmission of the infection among animal and men in the region.
Accordingly, this study recommended investigating of the T. gondii infection in definitive and other intermediate hosts in other
points of Khuzestan province, Southwest, Iran.