A study was conducted to determine the incidence cases of HIV/AIDS infection in Owerri West L.G.A. of Imo State, Nigeria, between Apirl 2007 and September, 2009. Data was collected from the HIV/AIDS co-ordination and control unit, Owerri General Hospital Umuguma, Owerri West Local Government Area of Imo State, Nigeria. It was observed that a total number of 17964 patients made up of 1110 (16.3%) females and 6954 (38.7%) males were diagonized on HIV/AIDS infection statistical analysis shows that only 1462 (8.1%) patients were infected with HIV/AIDS. From this number, 1218 (83.3%) patients aged 25 years and above were most infected with HIV/AIDS while 244 (16.7%) patients aged below 24 years were also infected. The incidence rate of HIV/AIDS infection was moderate at 8.1 while annual increase in HIV/AIDS infection was not statistically significant at p<0.05.