The HIV pandemic has ravaged mankind for over 3 decades. Gradually, incidence of new infections has declined globally due to increased prevention and control measures. In Southwestern Nigeria, there has been increased surveillance activity to complement control measures in recent years. We report here findings of the latest HIV surveillance revealing the prevalence of HIV in Abeokuta, Nigeria. About 744 subjects were tested for HIV seroconversion between January and March 2010. Screening tests were carried out using Determine™ HIV 1 & 2 (Abbott) and Chembio HIV 1& 2 Stat-Pak™ rapid test kits following manufacturer’s instructions. A seroprevalence rate of 11.7% (87/744) was determined with a significantly higher seropositivity in females (16.0%; 60/375) than males (7.3%; 27/369). Age group 16-45 years old was found to have the highest seroprevalence rate of 13.6% (70/514), followed by the age group 46 years and older with 11.0% incidence (12/109), and lastly, the age group 1-15 years old had the lowest HIV seroprevalence of 4.1%(5/121). HIV prevalence in outpatient was the highest, (12.5%; 33/264). This was followed by OPD having 15.0 (9/60), inpatient (Ward) subjects who had 11.0% seroprevalence (22/199) and others having 8.3% seroprevalence (14/168). The lowest HIV seroprevalence was recorded in Outreach visitations with only 2 positive cases (3.8% seroprevalence; 2/53). This current report shows a fairly high HIV seroprevalence rate in Southwestern Nigeria, with relatively high rate of HIV infections among the elderly. According to these findings prevention and control measures should thereby be stepped up, particularly at the local level with more attention toward sexually active adults and the elderly.