The research draws on a feminist political ecology perspective to demonstrate that agrarian restructuring and rural–urban transformation in Botswana offers women opportunities to renegotiate their marginalised positionality within the commercial urban agricultural sector in Greater Gaborone. Men and women participate in equal numbers, and both perceive of this sector as offering them new and accessible avenues for economic and social advancement. Although there is continuity of women's social and economic disadvantage relative to men from rural to urban contexts, women are actively making claims on land and capitalising on their traditional roles and responsibilities associated with poultry production. This negotiation of continuity and change in gendered positionality reflects and indeed suggests positive changes for women in urban Botswana, pointing specifically to the transformatory potential of urban agriculture despite existing constraints at the sectoral level. The research highlights the ways in which women are (re)defining their constraints, and seeking out alternative opportunities for empowerment and action. To this end gender remains an integral part of and key element to understanding agrarian restructuring and rural–urban transformation in Botswana.