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Citation Information

Type Journal Article - Science Journal of Public Health
Title Isolation, Identification and Determination of the Prevalence of Mycobacterium tuberculosis Complex among People Living with HIV in Kisumu County, Kenya
Author(s)
Volume 4
Issue 4
Publication (Day/Month/Year) 2016
Page numbers 359-365
URL http://chuka.ac.ke/publications/PeopleLivingwithHIVinKisumuCountyKenya.pdf
Abstract
Mycobacterium tuberculosis complex are important pathogens to man and causative agents of tuberculosis.
Tuberculosis is one of the diseases that continue to be a public health concern in the world. Earlier, tuberculosis was considered
controlled, but with emergence of HIV and AIDS, the disease has been exacerbated, with the highest incidences being in subSaharan
Africa. Kenya ranks 15th and 5th in the World and Africa respectively. The World Health Organization indicates that
there were 300 TB cases per 100,000 people in 2011. Information on the identity of the Mycobacterium tuberculosis complex
and the prevalence of pulmonary tuberculosis in HIV positive patients seeking treatment in Kisumu County is limited. In this
study, we isolated and identified the Mycobacterium tuberculosis complex and also determined the prevalence of tuberculosis
in HIV patients seeking treatment in Kisumu County. A cross-sectional study was conducted between December 2013 and June
2014. The study engaged, 379 HIV positive patients suspected of TB infection who gave sputum samples. The sputum samples
were then decontaminated, concentrated, liquefied and neutralized before being cultured in liquid media using MGIT 960
tubes. The culture positive MGIT tubes were sub cultured in Brain Heart Infusion Agar (BHIA) before microscopic
examination of the culture using ZN smear for Acid Fast Bacilli and identified using Genotype MTBC. In the study, 130
(34.3%) of the 379 suspected TB patients were diagnosed positive for pulmonary TB by MGIT culture. A significantly greater
number of males were diagnosed with pulmonary tuberculosis compared to females (57.7% and 42.3% respectively; χ2
=
1.0342, df = 1 P < 0.05). Further, the study revealed that TB prevalence decreased steadily with age, with the youths being at
greater risk of becoming active patients. In the age category of 26-35 years, prevalence was 36.92% while, for age category
above 55 years it was 6.15%. Based on the results, the etiological agent of tuberculosis in Kisumu County is Mycobacterium
tuberculosis, the prevalence of infection was higher in males than in females and the prevalence rate to infection was highest
among the youth. There is need for rapid and accurate diagnosis of active TB particularly in HIV-positive patients. Also, TB
awareness and control programme should mainly be directed towards the youths by the all the stakeholders in the Public
Health sector.

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