Aim To determine allele frequencies’ distribution for 15
short tandem repeat (STR) loci in a population sample of
1910 unrelated individuals from the region of Wallachia,
South Romania.
Methods DNA was isolated using Chelex 100 method and
an adapted version of AGOWA mag DNA Isolation Kit Sputum.
Polymerase chain reaction amplification was done
using AmpFlSTR Identifiler kit (D8S1179, D21S11, D7S820,
CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338,
D19S433, vWA, TPOX, D18S51, D5S818, and FGA). For DNA
typing, ABI PRISM 3100 Genetic Analyzer was used. Genetic
parameters of forensic interest were calculated and comparisons
with geographically close populations were performed.
Results With the exception of vWA locus (P=0.001), no
other significant deviations from Hardy-Weinberg expectations
were found. Single locus comparisons with data
on geographically close populations showed significant
differences between the population of Wallachia and the
population of Bucharest area, Greece, Turkey, Italy, Hungary,
Belarus, and Poland, but no differences were found from
the population from Croatia and Serbia.
Conclusion According to 15 analyzed STR loci, the population
of Wallachia region was found to be genetically more
similar to Slavic populations of Croatia and Serbia than to
other surrounding populations.