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Type Journal Article - BioMed research international
Title Identification of Virulence Factors Genes in Escherichia coli Isolates from Women with Urinary Tract Infection in Mexico
Author(s)
Volume 2014
Publication (Day/Month/Year) 2014
URL http://downloads.hindawi.com/journals/bmri/2014/959206.pdf
Abstract
E coli isolates (108) from Mexican women, clinically diagnosed with urinary tract infection, were screened to identify virulence
genes, phylogenetic groups, and antibiotic resistance. Isolates were identified by MicroScan4 system; additionally, the minimum
inhibitory concentration (MIC) was assessed. The phylogenetic groups and 16 virulence genes encoding adhesins, toxins,
siderophores, lipopolysaccharide (LPS), and invasins were identified by PCR. Phylogenetic groups distribution was as follows:
B1 9.3%, A 30.6%, B2 55.6%, and D 4.6%. Virulence genes prevalence wasecp 98.1%, fimH 86.1%, traT 77.8%, sfa/focDE 74.1%, papC
62%, iutA 48.1%, fyuA 44.4%, focG 2.8%, sfaS 1.9%, hlyA 7.4%,cnf-1 6.5%,cdt-B 0.9%,cvaC 2.8%, ibeA 2.8%, and rfc 0.9%. Regarding
antimicrobial resistance it was above 50% to ampicillin/sulbactam, ampicillin, piperacillin, trimethoprim/sulfamethoxazole,
ciprofloxacin, and levofloxacin. Uropathogenic E. coli clustered mainly in the pathogenic phylogenetic group B2. The isolates
showed a high presence of siderophores and adhesion genes and a low presence of genes encoding toxins. The high frequency
of papC gene suggests that these isolates have the ability to colonize the kidneys. High resistance to drugs considered as first choice
treatment such as trimethoprim/sulfamethoxazole and fluoroquinolones was consistently observed.

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