To observe the prevalence rate and pattern of drug sensitivity of bacterial
infection among urinary isolates from rural part of West Bengal, India. A total of
9,763 urine samples of clinically suspected UTI were collected. The samples
were inoculated on Blood agar, Mac Conkey's agar, and cystine lactose
electrolyte deficient (CLED) agar and incubated at 37 °C for 24 hr and extended
up to 48 hr in cases of no growth of any organism. Antibiotic susceptibility
testing against the isolated aerobic bacteria was performed by Kirby Bauer’s disc
diffusion method. Altogether 9,763 urine samples from tribal areas were
collected for this study from January, 2013 to December, 2015. Total 2,710
(27.75%) samples were shown to be positive for growth of any bacteria. The
dominant aerobic microorganisms isolated as the causative agents were E. coli
(64.65%), Klebsiella spp. (16.27%), Pseudomonas aeruginosa (4.09%), Proteus
spp. (3.21%), Staphylococcus aureus including coagulage negative Staphylococci
(6.46%), Enterococcus spp. (1.00%) and other gram negative bacteria (4.32%). E.
coli was the most common gram negative aerobic pathogen of UTI isolated in
tribal population. In-vitro antibiotic susceptibility testing showed that the gram
negative bacteria were sensitive to aminoglycosides (amikacin) and carbapenems
(meropenem), while the gram positive isolates were sensitive to norfloxacin,
gatifloxacin and levofloxacin. Isolation of the aerobic microorganisms from UTI
patients of tribal population in rural part of West Bengal revealed E. coli as the
most common bacterium in significant bacteriuria and amikacin is the most
effective drug against the clinically isolated pathogens.