Background: Applying advances in nutritional science to musculoskeletal medicine remains challenging, although in vivo data are lacking, the inhibitory effects of omega-3 on inflammatory factors implicated in the pathogenesis of osteoarthritis provide ample rationale for further investigation in humans with osteoarthritis. Aim: was to determine the effect of omega-3 fatty acids supplementation for ameliorating clinical signs of osteoarthritis. Materials & Methods: An intervention randomized pretest-posttest study was carried out on 47 females aged 40- 65 years with osteoarthritis. Patients were interviewed about knee complaints, progression of the disease, medical and physical treatment. Body weight and height were measured, and knee was examined. Functional assessment was done using Lequesne´s functional index. Patients received a daily dose of 2000 mg fish oil for 3 months. Clinical and functional reassessment of the were done after three month from starting supplementation. Results: The majority of females had osteoarthritis in both knees and all of them complained of knee pain, swelling and joint disability. About three quarter (78.7%) of cases took NSAID and a lower percentage (6.4%) used topical treatment only and 14.9% used both. After omega 3 supplementations for 3 months many improvements have occurred in patients’ symptoms, signs and drug intake. The percent of cases that still have knee swelling and tenderness dropped from 100% to reach 27.6% and 36.1% respectively. Conclusions: Findings from this study are strongly suggestive that a daily dose of 2000 mg omega 3 PUFAs significantly inhibits inflammation and reduces arthritic symptoms within a treatment period of 3 months.