This paper explores that there is gender segregation in the Pakistan labor force market. To calculate this segregation data from Pakistan labor force survey 2009-10 has been used and Duncan index technique was used to calculate the segregation index. Researcher considered nine major professions to check the segregation index. After finding the segregation indices this paper finds the determinants of this segregation. This paper examines the impact of age, gender, training, province and education on segregation index. It was found that in the profession of the manager there was higher segregation comparatively. It was also observed that an increase in the educational segregation decreases. It is suggested that education should be improved so that the segregation may be reduced.