Methanolic extract of fifteen Nepalese medicinal plants were subjected to phytochemical screening,
antibacterial and antifungal properties. Human pathogenic bacteria Enterococcus specie, Escherichia
species, Staphylococcus species, Proteus mirabilis, Salmonella typhi and Salmonella paratyphi were used
to evaluate the antibacterial property while Candida albican was used to evaluate the antifungal property.
Phytochemical screening of the extract revealed the presence of alkaloids, tannins, saponins, flavonoids,
glycosides, terpenoids and steroids in different proportions among the plants. Most of the extracts
exhibited the selective antibacterial activity. The extracts showed highest antibacterial activity
against Enterococcus species (20.8±0.72 mm) followed by Escherichia coli (19.37±0.35) by plants
Clematis buchananiana and Psidium guajava respectively. Antifungal screening of these plant extract
showed that only three plants Solanum xantocarpum, Jasminum humile and Aegle marmelos were slightly
active against Candida albican.